Question: miRNA annotation doubt
0
gravatar for Kato
5.5 years ago by
Kato50
Japan
Kato50 wrote:

Hi,

I'm working with the annotation of Illumina reads against mirBase and I have the reads counts. However this annotation gives me two alignments for the two types of miRNA (5p and 3p), however, when I want to compareĀ  my result with published result I found that in the article they don't use this discrimination and the count only the miRNA.

They sum the two result, 5p and 3p, or they choose only one, and why?

In the other hand, I read that first at all they make an alignment to the human genome and after that to the mirBase. Nevertheless, is it possible do the annotation directly from the trimmed fastq to the mirBase?

Regards.

annotation mirna count mirbase • 1.6k views
ADD COMMENTlink modified 5.5 years ago by Lorena Pantano360 • written 5.5 years ago by Kato50
1
In the article they might use the old annotation with or without a *. Conceptually you can distinguish between mir gene/ stem loop and mature miR based on the r and R.
ADD REPLYlink written 5.5 years ago by Georg Summer140

Good point, the change from the something* annotation to the current 5p/3p could easily be the cause of this.

ADD REPLYlink written 5.5 years ago by Devon Ryan94k
2
gravatar for Devon Ryan
5.5 years ago by
Devon Ryan94k
Freiburg, Germany
Devon Ryan94k wrote:

Well you'll need to align the fastq file to something in any case. What you could do if you really don't care if it's the 5' or 3' mature product is align to the hairpin.fa file from mirBase. Regarding what they did in some paper, it's pretty much impossible to know unless they explicitly state things (or you can download their raw data and figure out how they got their results from them).

ADD COMMENTlink written 5.5 years ago by Devon Ryan94k
0
gravatar for Lorena Pantano
5.5 years ago by
Barcelona
Lorena Pantano360 wrote:

Hi,

if this is miRNA analysis, the correct way to proceed is to differentiate between 5p and 3p. These two different small RNA that can come from the precursor have different function.

In that paper, maybe they used the old annotation, where the name is the main miRNA precursor that it could be 5' or 3'.

I recommend to use mirdeep2, or miraligner to get the counts of miRNAs, the second one is more directed to isomirs detection. Don't remember if mirdeep2 can use only mirbase, but miraligner uses only that. There are other onlines tools, like mirnabench that will do the job for you.

Just make sure ending with a counts for the mature miRNA if you want to have some functional information. If you don't, you can map directly with the precursor, but this are rare cases.

cheers

ADD COMMENTlink written 5.5 years ago by Lorena Pantano360
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 972 users visited in the last hour