We wish to replace microarrays with RNA-seq..we've seen papers published that specify that technical quality is quite repeatable, and thus technical replicates are unnecessary. We have also seen publications that have used RNA-seq with no biological replicates to generate results showing gene signature differences. If we are to replace microarrays with RNA-seq, is it bad practice (I know there's publications, but still...) to run the exoeriment without biological replicates? It becomes much more expensive if we had to run these in duplicate, so it's a huge difference, and we wish to know how we should proceed and what things we need to think about here. Any input would be greatly appreciated. Thank you very much.