Aligning old SAGE tag-sequencing data to the latest reference genome
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9.5 years ago
nash.claire ▴ 490

Hi everyone,

I'm kind of new to the field of bioinformatics and I was wondering if anyone could help me.

I have some previously generated LongSAGE tag sequencing data that I would like to re-analyse in order to update it with the most recent reference genome. I have some experience in using the program GeneProf in order to analyse next generation tag-sequencing data but I was hoping to do something similar but using our old SAGE tag data too as a comparison. Can anyone recommend a program that can do this for me? GeneProf doesn't seem to like to do it for me (unless I'm doing something wrong which is also possible).

At the end of the day, whether it's SAGE tag sequences or next gen tag sequences, they're all still just short DNA sequences that need aligning to the genome. They can't be that different in how they're analysed right???

Thanks!
Claire

sequencing alignment • 1.4k views
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