Respected Sir/ Madam,
I am Neha Goel from India. I am working on transcriptome sequence data from 454seq. I have 10Mbps data, I preprocessed (Quality filter/ Quality Trimming) and obtained the filtered file . For Denovo assembly I chase the velvet-Oases. I installed the velvet and it gave the contig.fa and Sequences.fa, Now I ran the command for Oases.
./oases 'directory name/' -merge yes -scaffolding yes -min_trans_lgth 200
But I get the transcriptes.fa with out any data (0 bytes).
I am not getting why is it so??? I ran the previous commands :
velveth dir 31 -fastq -short reads.fq velvetg dir/ -read_trkg yes
I also have single read Roche data. Now Please guide me what should i do??