I have been reading paper "a practical method for the discovery of genomic rearrangements at breakpoint resoultion". and while reading, there is some sentence that I can't understand. It is about 5' and 3' coordinate and I thought that the author might be not write the process in detail but anyway I want to understand it so I am appreciated if you look these sentence for me. and below is the sentence.
"In our example, the closest exon of each discordant read is used to cluster discordant reads into distinct gene-gene groups. For every group, a genomic region Ri is defined for each gene by taking the minimum of all 3' coordinates in the cluster and the maximum of all 5' coordinates in the same."
In this sentence, I approximately understand why minimum of 3' coordinate and maximum of 5' coordinate but I can't understand fully with concept. I cannot totally imagine the picture of it.
Can anybody give me a more Intuitive answer?