pooling 16sRNA samples in UPARSE pipeline
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7.1 years ago
Quak ▴ 380

One of the steps in the 16sRNA pipeline is concatinating/pooling reads from different samples (in order to study the beta diversity).

For example I can use the cat command as following

cat sample1_R1.fastq sample2_R1.fastq sample3_R1.fastq >> tot.fastq

and go ahead and remove chimera, label OTUs and etc. However, I wonder how can I look back and figure out which sample has came from what subject ?! Of course I can make a script to look up reads and assing them to subject; but I thought there might already be something exists

uparse 16sRNA • 1.6k views
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