pooling 16sRNA samples in UPARSE pipeline
Entering edit mode
7.9 years ago
Quak ▴ 480

One of the steps in the 16sRNA pipeline is concatinating/pooling reads from different samples (in order to study the beta diversity).

For example I can use the cat command as following

cat sample1_R1.fastq sample2_R1.fastq sample3_R1.fastq >> tot.fastq

and go ahead and remove chimera, label OTUs and etc. However, I wonder how can I look back and figure out which sample has came from what subject ?! Of course I can make a script to look up reads and assing them to subject; but I thought there might already be something exists

uparse 16sRNA • 1.8k views

Login before adding your answer.

Traffic: 881 users visited in the last hour
Help About
Access RSS

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6