MEGAN, fasta file without sequence length in ID lin

Question

[cara78]

Hello,

I am planning to use MEGAN. I am currently reading around to prepare. I have come across that MEGAN takes fasta files but have to have the sequence length in the ID line. Mine don't. I asked a friend who did this type of analysis some time ago and said it wont matter. But I just want to double check that MEGAN will take my fasta file without the sequence length. Did anyone every come across similar problem before? I am doing it from the command line.

Thanks

[iraun]

I've never use MEGAN but... you can just make a little dataset with sequence IDs with and without sequence lengths and see what is going on?

Answer 1

[Istvan Albert]

AFAIK there is no such limitation for MEGAN - it would kind of be a really odd limitation - after all the sequence is right there as well and the tool needs to read anyhow it so it will know how long it is