MEGAN5 software: anyone know how to correct "no reads found" message when trying to upload megablast output from Galaxy?

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9.5 years ago

Ina Quest ▴ 10

Its driving me crazy but I cannot seem to be able to load my blast output and my reads all at once. It keeps saying "No reads found". Does MEGAN not work with megablast outputs?

The fasta file for the reads is the exact same one I used to do a megablast on the Galaxy platform, and the blast output is the standard output. I did filter it down to hits with a >=90% identity and a min of 100bp. The megablast output is downloaded as a tabular file but I have changed it to blast, but maybe it doesn't like that.

two questions:

1. Has anyone else encountered this problem? Any ideas on how to go from Galaxy to MEGAN?

2. Is it possible to upload the reads at a later point? and how?

Thanks everyone for your time and help.

Ina

metagenome reads MEGAN GALAXY megablast • 2.8k views

ADD COMMENT • link updated 9.3 years ago by Biostar 20 • written 9.5 years ago by Ina Quest ▴ 10

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Which output format are you getting from megablast? Tabular? XML? Plain text? Which format does MEGAN expect to be given the BLAST results?

ADD REPLY • link 9.3 years ago by Peter 6.0k

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