Finding Integrative and Conjutative Elements in genomes (ICE)

Question

[robjohn70000]

Hi,

I blasted my contig sequences against a database of ICE, but not sure what other filtering parameters to use apart from E-value<1e-5 and percent ID=100% that can help me to pick out the best hits as I still have thousands of hits in my blast output. I thought of applying further filtering based on ICE sequence lengths, but not sure what to use. Does anyone know if a particular sequence length (such as 1500 nt for 16SrRNA) applies to ICEs. I have tried (alignment/ICE seq len)*100 and this was not really helpful.

Any advice will be appreciated.

Thanks