I try to do the diff analysis by DESeq on two samples coming from two different condition without replicate
I run the script of DESeq and when I give the library size of my sample, the size factor is 1 for both conditions
libsizes <- c( Cond1 =80040653, Cond2 =360265740) cds=estimateSizeFactors(cds) sizeFactors(cds) Cond1 Cond2 1 1
I don't understand this estimation?
when I don't give the libsizes, it become
sizeFactors(cds) Cond1 Cond2 1.7320508 0.5773503
Could you please explain for me this issue?
Thanks in advance Sara