Question: Cufflinks run showing “Multihit not found”
0
gravatar for mjoyraj
4.4 years ago by
mjoyraj50
Taiwan
mjoyraj50 wrote:

I run my RNA seq data for alignment and assembly with the below mentioned options. The cufflinks run is repeatedly showing "Multihit not found". Is this a serious issue.

tophat -p 12 -G Genes.gtf -o tophat_RABM --max-multihits 2 Genome R1.fastq R2.fastq

cufflinks -p 8 -o cufflinks_RABT --max-bundle-frags 1000000000000 --multi-read-correct tophat_RABM_/accepted_hits.bam

rna-seq alignment assembly • 1.2k views
ADD COMMENTlink written 4.4 years ago by mjoyraj50
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 1775 users visited in the last hour