I am confused about the meaning of clustering of transcriptome sequence and assembly of transcriptome sequence.
Based on what I understand, assembly means overlapped reads join to each other in order to form a full or partially sequence of mRNA.Am I right?
clustering is categorizing the set of homologous gene(expressed mRNA in transcriptome data).Am I right?
clustering should be done after assembly but why we do clustering for transcriptome data?
it might seems silly , but what is different between clustering and Multiple sequence alignment ? both shows the sequences similarity.
English is not my first language, so please excuse any mistakes.
Thanks in forward.