Merry Christmas and Happy New Year,
Hi,I am new to this research.I am trying to understand how to fetch intron location.I have get gene location and exon location from NCBI, but I am still confused about exon location. My question could be shown as follows:
Structure1: 5'UTR -> exon -> intron ->exon->intron->exon-> 3'UTR
Structure2: 5'UTR-> intron->exon->intron->exon->intron->3'UTR
Q1: when I only consider intron location, whether I do not consider 5' or 3' UTR as intron? What I mean is 5'UTR or 3'UTR is not the same as intron.
Q2:As we learned, Structure1 is usually structure for gene.However, is that possible in Structure2? 5'UTR is adjacent with intron, but not exon.From NCBI .gff download file, I only could get gene location and exon location. If Structure2 is possible, how do I define intron location without 5'UTR location?
Thanks for your help.