Question: primer design function for perl program
0
gravatar for J.F.Jiang
4.7 years ago by
J.F.Jiang750
China
J.F.Jiang750 wrote:

Hi all,

Although there are well enough online program for primer design, such as web tool from illumina and life.

To develop several specific primers, I want to know is there any source that I can get to confirm the function of primers, such as Complementarity, dimer,Stability...?

Thanks.

 

primer perl • 2.0k views
ADD COMMENTlink modified 4.7 years ago by Jean-Karim Heriche20k • written 4.7 years ago by J.F.Jiang750
1
gravatar for Jean-Karim Heriche
4.7 years ago by
EMBL Heidelberg, Germany
Jean-Karim Heriche20k wrote:

Have you tried the Primer3 program ? BioPerl has a wrapper for it. I think BioPython also has one.

ADD COMMENTlink written 4.7 years ago by Jean-Karim Heriche20k

The Perl wrappers are in: Bio::Tools::Primer3 - search.cpan.org and Bio::Tools::Run::Primer3 - search.cpan.org

ADD REPLYlink modified 4.7 years ago • written 4.7 years ago by Michael Dondrup46k

Thanks, Michael.

I used both of the modules for perl you provided before, however, even I looked into the pm files for details, they did not provide the function for the primer selection, such as Complementarity, dimer...

ADD REPLYlink written 4.7 years ago by J.F.Jiang750

Thanks for your comment.

I can use the primer3 or other newest tools for primer design.

However, I want to know if there is any perl function or other languages to define the function for primer selection.

That's what I want.

Thanks.

ADD REPLYlink written 4.7 years ago by J.F.Jiang750
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