Question: (Closed) Convert FASTA file into FASTQ file
1
gravatar for suhalsuhaimi
5.6 years ago by
suhalsuhaimi30
United States
suhalsuhaimi30 wrote:

hi all,

can anyone please help me, how to convert my fasta file into fastq file.

Any other tool or open source to do this conversion?

fastq fasta • 26k views
ADD COMMENTlink modified 5.6 years ago by Matt Shirley9.4k • written 5.6 years ago by suhalsuhaimi30

Hello suhalsuhaimi!

Questions similar to yours can already be found at:

We have closed your question to allow us to keep similar content in the same thread.

If you disagree with this please tell us why in a reply below. We'll be happy to talk about it.

Cheers!

PS: Please see existing similar questions
ADD REPLYlink written 5.6 years ago by RamRS28k

Thank. I already to the link. And finally i can convert it. But it don't have quality score. My velvet software did not produce any result when i try to assemble this sequence?

Any other solution?

 

ADD REPLYlink written 5.6 years ago by suhalsuhaimi30

The post contains details on that too - check out Manu's answer in it.

ADD REPLYlink written 5.6 years ago by RamRS28k
1
gravatar for Matt Shirley
5.6 years ago by
Matt Shirley9.4k
Cambridge, MA
Matt Shirley9.4k wrote:

See answers here: convert FASTA into FASTQ using linux

Note that FASTQ files carry quality scores for each base call, so unless you have QUAL files for each FASTA file, you will end up adding extra (made-up) information during the conversion process.

 

ADD COMMENTlink written 5.6 years ago by Matt Shirley9.4k

 Thank for the info. But then how to i get the QUAL file for my fasta sequence.

 

 

ADD REPLYlink written 5.6 years ago by suhalsuhaimi30

You would have to receive these with your FASTA files - for instance if your sequences are from Sanger sequencing you can usually get quality scores.

ADD REPLYlink written 5.6 years ago by Matt Shirley9.4k
Please log in to add an answer.
The thread is closed. No new answers may be added.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 1585 users visited in the last hour