I have an unstranded RNA-seq data. I have a question about this seq type:
if several reads were mapped onto an intergenic region, it indicates presence of a noncoding RNA; however, which strand this ncRNA is derived from? To my understanding, in the unstranded library construction method, both sense and antisense strands are produced before sequencing, so which strand the reads come from?
Any of your answers will be appreciated. Thanks a lot!