When using fastqc to assess the quality of small RNA-seq data, what is a good results? (It's seems that the quality is not good for all the data)
How to deal with biological repeats (e.g. 3 fastq files)?
Is it ok to merge them first? If so, how to merge them, any tools? Can I just use
$cat A_1_R1.fastq A_2_R1.fastq A_1_3_R1.fastq > A_merge.fastq?
What is the difference bewteen sRNA-seq and RNA-seq when filtering?
For now I just try to trim the adapter and remove reads form repeat region of genome.
I think really need some tutorial for sRNA-seq anaylsis....
Do any biostarers have some tools or pipelines to share for sRNA-seq analysis?