What Is Score Bits In Blast?
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Entering edit mode
10.1 years ago
Manju ▴ 50

i want to konw that what does it mean score and bits in local blast output from the following example?'.

Score = 38.2 bits (19), Expect = 0.11
 Identities = 22/23 (95%)



Query: 1      cttaacttcagctacctcgagta 23
          <br>|||||||||||||||||| ||||<br>
Sbjct: 732552 cttaacttcagctacctccagta 732574
blast scoring • 33k views
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8
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10.1 years ago
Nikolay Vyahhi ★ 1.3k

See this description of the bit score on NCBI.

Also from this NCBI page:

The bit score gives an indication of how good the alignment is; the higher the score, the better the alignment. In general terms, this score is calculated from a formula that takes into account the alignment of similar or identical residues, as well as any gaps introduced to align the sequences.

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Thank you, Nikolay, for the "obvious" answer. This is a legitimate question, of course, but could have easily been answered by Manju by simply looking at the extensive and useful help pages and other documentation provided on the NCBI pages.

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10.1 years ago

A bit is a measure of information content. Bites, like bytes, are units of measure for digital information, in this case, with regard to the alignment and hence the similarity of the two sequences.

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