Question: Comparing Genome Derived And Cdna Derived Protein Sequences
gravatar for Woa
9.0 years ago by
United States
Woa2.8k wrote:

I've two sets of protein sequences for a particular organism for which the genome sequence is available to us.

One of the protein sets is derived from comparing the genome with all the proteins of a related organism using a protein-genome alignment program.

The second protein set was derived from a translated cDNA library from the same organism.

I wish to find out the equivalent pair of proteins(supposedly almost identical) from these two sets.

Can anybody suggest any good method for this? Can I use 'reciprocal blast best hits' method?

Thanks in advance


comparison protein blast • 2.3k views
ADD COMMENTlink modified 5.4 years ago by h.mon31k • written 9.0 years ago by Woa2.8k

However things went not so good. Many of the cDNA proteins were fragments and they are yielding erroneous results. Can anybody suggest any alternative way to bypass the problem?

ADD REPLYlink written 9.0 years ago by Woa2.8k
gravatar for Larry_Parnell
9.0 years ago by
Boston, MA USA
Larry_Parnell16k wrote:

Yes, the reciprocal or mutual best BLASTP hit approach will work. It may miss a couple of pairings, but will find in excess of 99%, in my opinion.

ADD COMMENTlink written 9.0 years ago by Larry_Parnell16k
gravatar for h.mon
5.4 years ago by
h.mon31k wrote:

Map both your predicted proteins and the cDNA against the genome, the overlaps will give you what you want. Spaln perform both alignments and it is quite fast, you can probably run it on your personal computer for your datasets.

ADD COMMENTlink written 5.4 years ago by h.mon31k

oh crap, sorry I am posting to a very old question. shame on me.

ADD REPLYlink written 5.4 years ago by h.mon31k
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