Chip Dna Deep Sequence
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12.0 years ago
Yang Yang ▴ 50

Hi, everyone, I am posting this question for my friend. He is analyzing his CHIP DNA solid deep sequence data, and find out that near 80% reads can not be mapped to the human genome. We are wondering if this high percentage unmapped reads is normal in CHIP DNA deep sequence or there may be something wrong with his result.

solid chip-seq • 3.2k views
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12.0 years ago
Gue Su Chang ▴ 50

Your 20% mapping yield looks like low for normal ChIP experiment, even for human. Several factors can reduce this mapping yield. I am wondering which kind of ChIP was used in your case. That is, which kind of proteins was ChIPed?

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12.0 years ago
Zhenhai Zhang ▴ 170

Hi there,

We have done numbers of SOLiD sequencing run on yeast samples. Normally there are only 30-40 percent of total tags can be uniquely mapped back to yeast genome.

What I would recommend is do it on solexa. You get much higher quality tags.

cheers,

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12.0 years ago

I recall that our first samples that we ran on the Solid sequencer have had bad performance. Not quite an 80% loss but around 40%-60% reads were unmappable (yeast). Some other lab members will hopefully chime in with more details.

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