Entering edit mode
11.5 years ago
Paul ▴ 760
I'm trying to calculate which genes in my microarray samples are expressed above background (produce the dabg.summary.txt file). I've issued the following command in the linux terminal:
apt-probeset-summarize -a dabg -p supportFiles/HuEx-1_0-st-v2.r2.pgf -m supportFiles/HuEx-1_0-st-v2.r2.dt1.hg18.core.mps -c supportFiles/HuEx-1_0-st-v2.r2.clf -b supportFiles/HuEx-1_0-st-v2.r2.genomic.bgp -o ./OUT_DABG_genomicBg *.CEL
Which gives the following strange error:
Processing Probesets............ FATAL ERROR:No background probes found for GC bin 24 for probe with id: 1025125 Perhaps there is no probe with GC count 24 in bgp-file: supportFiles/HuEx-1_0-st-v2.r2.genomic.bgp
The strange thing is when I use the antigenomic probeset file as background (HuEx-10-st-v2.r2.antigenomic.bgp) I get no error at all, but I need to use genonic (HuEx-10-st-v2.r2.genomic.bgp) to reproduce somebody else's results.
I've no idea what's going on here and found nothing online so any pointers would be a massive help.