Question: why are all the genes on the positive strand after annotation using Basys annotation server?
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gravatar for samuelksm
5.0 years ago by
samuelksm0
Ireland
samuelksm0 wrote:

i annotated my bacterial sequence with basys annotation server the annotation came back with 7500 gene but all the genes appear to be on the positive strand, so i decided to re annotate with RAST this time 4500 genes came back both on positive and negative strands. i wanted to work with the basys file but why are they all on the positive strand, can any one explain to me what is happening?

sequencing • 1.4k views
ADD COMMENTlink written 5.0 years ago by samuelksm0

Perhaps this from the docs: https://www.basys.ca/server1/basys/cgi/howto.pl

"The defline must be in the form >identifier:cstart-end name, where: specifies that the coding sequence is on the complementary strand. If the coding sequence is on the direct strand, just omit the c character."

ADD REPLYlink written 5.0 years ago by Istvan Albert ♦♦ 82k

Thanks Albert, i contacted the Basys annotation server workers and they just replied the server contained a bug, but they had fixed so i just have to re annotate my sequences.

ADD REPLYlink written 5.0 years ago by samuelksm0
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