biological replicates analysis
0
0
Entering edit mode
9.4 years ago
schelarina ▴ 50

Hello,

I have two biological replicates for sRNAseq and I am not sure how I can analyze them. I am not interested in doing differential expression analysis since this samples were not subjected to any treatment. Would it be correct to calculate the average of the coverage per position between the two replicates?

Thanks for your answers

RNA-Seq • 2.2k views
ADD COMMENT
4
Entering edit mode

What was the goal of the experiment? You don't normally start a project saying, "hey, let's sequence some stuff just for the heck of it!"

ADD REPLY
0
Entering edit mode

sRNAseq is what?

not subjected to which treatment?

which species?

which cell line?

ADD REPLY
0
Entering edit mode

sRNAseq is smallRNAseq

species: Arabidopsis

for treatment I mean any kind of stresses (biotic or abiotic)

ADD REPLY
0
Entering edit mode

Okay,

You could use HT-seq count on individual samples and then probably mean of counts could be assigned to each gene

ADD REPLY

Login before adding your answer.

Traffic: 2841 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6