I have aligned NGS six runs of a sample using BWA (galaxy platform). Then I merged them into one BAM file. Next I sliced the region of interest using SliceBAM in BED tools. After that I merged sequences in the resulting file using Merge command in Operate on Genomic Intervals. So the final result is a BED file. How can I convert this BED file into a FASTA file?
Question: Are these steps correct? Galaxy
4.9 years ago by
rus2dil • 20
rus2dil • 20 wrote:
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