I have a bam file with ssRNA-Seq data. i was viewing it using bamview.
I blasted one of the both positive and negative strand sequences on NCBI using blast and gives a positive strand result for both. For positive read beginning of the gene and for the negative read at the end of the gene. I get the position but shouldn't the reverse strand read be +/- when blasted?
Could anyone please explain?
Thank you so much!