Question: Ancestral Sequence Reconstruction from Multiple Sequence Alignment using Mesquite
1
gravatar for anuragm
5.9 years ago by
anuragm130
India
anuragm130 wrote:

I have a nexus tree file and a multiple sequence alignment(MSA) in nexus format. I am interested in ancestral sequence reconstruction using Mesquite. I had been following this tutorial : http://phylo.bio.ku.edu/slides/lab9-MesquiteAncStatesBisse/09-Mesquite.html#Part_B:_Estimating_ancestral_states
 

But after the step that says convert the tree display to 'balls and sticks' and Branches proportional to lengths, I am not seeing a Ancestral Reconstruction option when I click the Analysis tab in the trees window.

A difference is that my data is a sequence alignment whereas the data in the example on the webpage is categorical. What is it that I am doing wrong ? Is there a way to do it with a MSA ?

ancestral state mesquite • 2.6k views
ADD COMMENTlink modified 5.9 years ago by rmccloskey220 • written 5.9 years ago by anuragm130
0
gravatar for rmccloskey
5.9 years ago by
rmccloskey220
Canada
rmccloskey220 wrote:

As far as I know, Mesquite has support only to reconstruct a single discrete state at a time. So if you want to reconstruct the entire ancestral sequence, you would have to encode each position as a separate state (like the categorical example data), then reconstruct them all separately. Obviously this is quite a hassle, so you might try using a different tool. In the past I've used HyPhy to do ancestral sequence reconstruction: there is a code example here but documentation is fairly scarce. Alternatively, FastML can do ancestral state reconstruction.

ADD COMMENTlink written 5.9 years ago by rmccloskey220
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