I am trying to set the number of maximum hits to 5, so that the procedure can finish sooner, but I still get 100s of hits found.
# TBLASTX 2.2.29+ # Query: Locus_40_Transcript_185/186_Confidence_0.224_Length_4778 # Database: ../../Genome/Genome # Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, q. start, q. end, s. start, s. end, evalue, bit score # 714 hits found
I am running:
tblastx -db ../../Genome/Genome -query all_merged_k125.fa -evalue 1e-10 -outfmt 7 -out tblastx/all_merged_k125.fmt7 -num_threads 16 -max_target_seqs 5
Any idea why it's still reporting so many hits?