Question: How to apply RLM normalization to a protein expression matrix in R?
gravatar for tasjfasfankihj
6.0 years ago by
tasjfasfankihj10 wrote:

I have a combined dataset of 123 samples and 9,482 features (expression levels of antibodies from Invitrogen's ProtoArray v5.0).  Based on (, RLM normalization is the optimal method for my data.  What I need help with is figuring out how to implement it in R.

PAA package says it has it, but doesn't work and the only thing I can find online about it is someone's post asking for help with no response.

Is there a way to do it with Limma's normalizebetweenarrays()?

The authors of the article I linked above apparently used the MASS package, but I haven't been able to figure out how.

Can someone please explain how to do it? Example code would be really helpful if possible.

rlm normalization R • 2.0k views
ADD COMMENTlink modified 6.0 years ago by Mo920 • written 6.0 years ago by tasjfasfankihj10
gravatar for Mo
6.0 years ago by
Mo920 wrote:

I have never done this normalisation , have a look at this package , I found there is a function for RLM normalisation (page 8)

You can find some theory here

here is an example of RLM in MASS package

Page 127 of this as well

Hope it helps 

ADD COMMENTlink modified 6.0 years ago • written 6.0 years ago by Mo920
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