Weird output when trying to convert sam to bam!
1
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Entering edit mode
9.1 years ago
Parham ★ 1.6k

Hi,

I have mapped my reads using STAR. Then I am trying to convert the output SAM file to BAM using code below but I get a strange respond. Could somebody tell what I am doing wrong? Thanks in advance.

samtools view -Sb Aligned.out.sam aligned.bam
[samopen] SAM header is present: 6 sequences.
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[main_samview] random alignment retrieval only works for indexed BAM files.
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                                                                                            ^?[����*�D����bQ�Pe�8��jR�i?��6�?Z��VTD?!I#���*�R_&CKru˴?
�?I���`]��?�1E���9���i>�?��<��m                     ѧI���!D��+$?%1?f�?B�x�0.�Ӳzd�p���g�e�2؏=t�
                               �Y����^�:�5�l�0�6?��N���[�    ��F��???�?BC???%
sam samtools bam • 2.8k views
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1
Entering edit mode
9.1 years ago

You need to specify the output '-o (out.bam)' argument

samtools view -Sb -o aligned.bam Aligned.out.sam
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0
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Oops, sure I need to. Thanks a lot!

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