I know the formula for theoretical read depth is not quite applicable for RNASeq data, as previously explained very well in this Biostars post:
A: human transcriptome size
So my question is when "coverage per sample" is reported, such as in below, is it usually estimated based on the total size of the "genome" as the "target size"? Or is it based on other experiment-driven parameters for each facility?
(if I use genome size, I get numbers that are close to the reported sample coverage numbers).
I appreciate your feedback,