I am studying the evolutionary aspect of gene family. I want to build the structure of each family member and want to estimate, how much paralogs (family members) are related or distant from each other at structural level.
But I found very low query coverage of target (family member) with template i.e:
Member A: Identity: 30% Query Coverage:49%
Member B: Identity: 33% Query Coverage:49%
Member C: Identity: 31% Query Coverage:52%
Template is available for N terminal of target protein whereas no template is available for C terminal of target protein.
Query coverage and identity of N terminal with target is :
Identity: 35% Query Coverage:91%
I want to Know whether I can build a reliable protein structure for family members using an abinito approach. I am interested in construction of structure for whole protein.
I will be grateful