First of all hello to everyone, this is my first post and I hope you can help me.
I'm an amateur in bioinformatics and I'm working in microbiota. We use 454 technology by Roche. To analyze diversities, I use qiime, it was difficult for me but finally I got it! but now I want to continue to the next step, the functionality.
I used to work with the tools that WebMGA gave me (fraggenescan, COG and KEGG databases) but I knew MEGAN and it seems very easy and with many opportunities for somebody like me that is learning now.
Well, please, don't think I am a completely idiot, but I am completely lost. I have used my out_table.biom from qiime into Megan and it's good, but I don't get new results, as I can't access to the COG and KEGG analysis.
So I tried to BLASTX my files, but it's impossible to use it (in fact, I am not sure if the files I am introducing are the correct), and I tried diamond, but I have few free space in my computer, and the analysis is killed in the middle.
I am completely desperate.
Please, can anybody indicate me the steps that I need to follow?
Thanks in advance!