Question: Searching For Proteins That Have Similar Type Of Residues In Binding Pocket
7
gravatar for Flow
9.0 years ago by
Flow1.5k
Flow1.5k wrote:

I am working with some protein that has 6 polar basic residues on its binding site. I would like to find similar proteins on PDB (or other database) that have this type of residues (polar basic residues (ARG or LYS or HIS)) at similar 3D positions (given some threshold). It would be something similar to ligand pharmacophore similarity search, but for parts of proteins. How can I perform this query?

PS: Given the unavailability of such software, I am thinking about developing a GPU based software that would scan whole PDB database for finding related proteins given user's input. If somebody is interested, we could collaborate. This is serious, please contact me.

protein database search motif • 2.8k views
ADD COMMENTlink modified 9.0 years ago by Khader Shameer18k • written 9.0 years ago by Flow1.5k
5
gravatar for Pawel Szczesny
9.0 years ago by
Pawel Szczesny3.2k
Poland
Pawel Szczesny3.2k wrote:

In the past I have used SuMo by Martin Jambon (also a BioStar user - Martin, where are you? :) ). It will require some modification of your PDB file so you can easily select which residues you want to take into account (for example by marking required residues as belonging to chain X, etc.). See the server for details.

Alternatively, you could try to download one of many programs designed to analyze protein structures for binding pocket similarity (ten or so are cited in the introduction of this short paper: http://stat.fsu.edu/~jinfeng/publication/TIPSA_ACMBCB2011.pdf ) and then substitute program's database with your own definition of binding pocket. However, I can imagine it's not going to be the most straitforward way.

ADD COMMENTlink modified 13 months ago by RamRS30k • written 9.0 years ago by Pawel Szczesny3.2k

very interesting, I will have a look, please look at my question's update

ADD REPLYlink written 9.0 years ago by Flow1.5k
4
gravatar for Bilouweb
9.0 years ago by
Bilouweb1.1k
Saclay, France
Bilouweb1.1k wrote:

Hello,

Your question make me think of my application for 3d motif search in protein structures : RASMOT3D

In rasmot, you give a definition of a reference 3d motif which contains :

  • a pdb file
  • the residues involved in the motif
  • for each residues, the list of equivalents residues.

Given this reference motif, rasmot3D search through the pdb to find similar sites (geometricaly).

The webserver is a light version of the application, if you need, we can collaborate for a more complex search through the PDB.

ADD COMMENTlink written 9.0 years ago by Bilouweb1.1k

please give me your email, if possible, thanks

ADD REPLYlink written 9.0 years ago by Flow1.5k

gcollet at ouvaton.org

ADD REPLYlink written 9.0 years ago by Bilouweb1.1k
3
gravatar for Khader Shameer
9.0 years ago by
Manhattan, NY
Khader Shameer18k wrote:

I would approach this problem from the perspective of sequence-structure analysis. I will use pattern search using amino acids and 3D spatial search to develop such a tool.

  1. I will compile binding site data from public resources of protein binding sites. Both sequence data (residues + structural positions) and structural data (full-PDB files) will be compile in to a database

  2. I will apply filtering conditions (for example classification of amino acids (polar residue, hydrophobic, charge etc) to the sequence dataset and get initial list of proteins for structural analysis

  3. Once you did the filtering, you can define a distance shell around c-beta atoms and compute distances between active site residues. You can use K-d tree or similar data structure for implementing such a program on CUDA-type architectures.

  4. You may use such distance shells to define and identify related structures. I think active site residues will be close (<= 7 Angstroms in distance).

I have used similar concepts of distance-shell searches to define higher order residue interactions in protein structures.

PS. How to contact you ? No contact details in your profile :) !

ADD COMMENTlink modified 9.0 years ago • written 9.0 years ago by Khader Shameer18k

werner.schroedinger at googlemail dot com

ADD REPLYlink written 9.0 years ago by Flow1.5k

Thanks flow, you've got a mail.

ADD REPLYlink written 9.0 years ago by Khader Shameer18k
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 853 users visited in the last hour