Multiplexed 454 Data Analysis
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13.1 years ago
User 3466 ▴ 10

I'm a newbie and I'd like to know how to start doing analysis of multiplexed 454 data. I know its wide question but I really don't know where to start?

next-gen sequencing • 3.1k views
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Do, or do not. There is no try. :)

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Your sequencing provider should demultiplex the data for you, assuming you used the standard Roche MID barcode system.

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13.1 years ago

check out the mothur package, it has 454 flow trimming and demultiplexing features that are very handy and usable even if your data has nothing to do with metagenomics

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thanks i will try with that

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12.9 years ago
Johnny Brown ▴ 140

Qiime may be useful to you. Particularly handy is the MacQiime all-in-one install for apple computers.

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