how can I use Lumi package for GSE31699 file?
0
1
Entering edit mode
9.1 years ago
SCA ▴ 10

I would like to use lumi package for normalize to http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE31699

I read csv file and get a matrix file. I tried to use the steps in http://www.bioconductor.org/packages/release/bioc/vignettes/lumi/inst/doc/lumi.pdf but it doesn't work.

My questions:

  1. How can I normalize to the my matrix file?
  2. What should I do for using this package?

Thanks in advance

R • 3.2k views
ADD COMMENT
1
Entering edit mode

Is there supposed to be a question here?

Edit: Ah, there's a question now, great!

ADD REPLY
0
Entering edit mode

Why do you want to normalize the matrix file ? Its already normalized -

"The data was preprocessed by Bioconductor lumi package (version 2.5.5). It was vst transformed and quantile normalized."

ADD REPLY
0
Entering edit mode

Thank you very much for your information. I am very new about bioinformatics and R so these knowledge is very helpful for me.

I look at there its useful for expression data analysis and I check my matrix and your saying data is not same. it is for just 1 sample but I have 36 samples. I have raw data matrix both methylation data and expression data so I have to normalized. actually doesn't matter be which packet lumi or others. I just want to normalized these matrix and than according to distribution (normal- non normal) I will use parametric tests. I will be most grateful, if you could help me.

ADD REPLY

Login before adding your answer.

Traffic: 2712 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6