Can anyone tell me what to do after getting a large no. of ORF of newly bacterial sequence which has not yet been submitted to NCBI. Please tell me how to analyze large no. of contigs for their functional annotation?
Hi, If its "newly bacterial sequence" and not available in database. You should Predict the CDs after all you need to blast to publicly available database such ad swissport or NCBI to get a functional annotation of all the gene.