I'm trying to download the appropriate databases or use with tophat-fusion-post.
I've installed blast+ on the Linux 14.04 LTS and this works. But then I need to download nt, human_genomic, human_genomic_transcript and other_genomic files from the NCBI website. And this is not working for me.
What I've tried so far:
- go to ftp://ftp.ncbi.nlm.nih.gov/blast/db in a Mozilla Firefox browser. This doesn't connect.
- wget -q ftp://ftp.ncbi.nlm.nih.gov/blast/db/nt.08.tar.gz But here I get the error: Failed: connection timed out, network is unreachable. This command was suggested on www.rocksclusters.org/roll-documentation/bio/5.4/blast_usage.html
- update_blastdb --verbose --force nt.08.tar.gz But again I get the message that it failed to connect because the connection timed out.
Anyone have any suggestions, because I don't know what else I can do. It looks like blast+ (update_blastdb) is working, but it cannot connect to the ftp.