Ideal coverage required for PacBio error correction using HGAP
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9.3 years ago
Felix Francis ▴ 600

What is the ideal PacBio coverage required for error correction (consensus polishing) using HGAP (by only using PacBio reads)? How effective is this approach in error correction?

How does this approach compare with hybrid error correction using Illumina short reads (assuming the reads are from a homozygous genome)?

PacBio HGAP error-correction Illumina • 2.9k views
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9.3 years ago

For HGAP only, recommended coverage would be 60x-100x (more repetitive genome, the higher). It seems to be very effective for small microbial genomes (you could end up with 1 or 2 contigs), it has issues for larger (and eukaryotic) genomes.

Comparison with hybrid approach will depend on many factors, most importantly genome size and complexity. For small microbial genomes, I wouldn't bother with Illumina and would do just PacBio and HGAP.

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Thanks for the information. So far for microbial genome we don't need any error correction with Illumina reads. Do you have any idea after HGAP we can get many repeated contigs. How to deal with that? You have to remove by visually or any other approach?

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