Question: Why would my the polyA selection RNAseq preparation method get much greater dispersion than the RNAseq data got from ribo-depletion library?
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gravatar for bibilong1111
4.2 years ago by
United States
bibilong11110 wrote:

 

I have RNAseq data got from both polyA selection and ribo-depletion library preparation, but the ribo-depletion library data showed much less dispersion. Their sequencing depth are the same.  Attached is the relative log expression plot and the first several sample are the polyA selection library. The data have not been normalized. 

rna-seq normalization • 1.2k views
ADD COMMENTlink written 4.2 years ago by bibilong11110

There are no figures...

What do you mean by dispersion? variance between replicates?

ADD REPLYlink written 4.2 years ago by Asaf5.7k
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