I obtained the job and need to identify presence of some bacteria in clinical samples by PCR with species/strain specific primers. The primers are already available in the lab, but the person who designed/find/order the primers were not available in the lab anymore and nobody knows the details. Therefore, I know primer sequences, the names of the strains, but I do not know the target (genome, 16S, ...), the size of the amplicon - actually I have no data to check the PCR result... I tried to blast them, but only one pair is found on the NCBI.
How to find the target sequences, can anyone help me. Are there are softwares or databases for blasting the bacterial genome/transcriptome?