Question: How to combine bisulfite sequencing data with Medip seq and/or MRE-seq
2
gravatar for Saad Khan
4.3 years ago by
Saad Khan360
United States
Saad Khan360 wrote:
I was wondering if anybody has done integration of data from two different sequencing methods for DNA methylation like bisulfite-seq and Medipseq.I would really appreciate if someone could suggest methods for the same. Thanks
ADD COMMENTlink modified 4.3 years ago by mark.ziemann1.2k • written 4.3 years ago by Saad Khan360
2
gravatar for mark.ziemann
4.3 years ago by
mark.ziemann1.2k
Australia/Mebourne/Geelong/Deakin
mark.ziemann1.2k wrote:

Do you have control/treatment data for both?

My suggestion would be to focus on promoter regions +/- 1kbp around TSS and quantify the methylation in each case, %methylation for BS-seq and counts per million for MeDIP-seq, then you can analyse differences with statistical test, merge datasets on gene accession number and perform correlation analysis.

ADD COMMENTlink written 4.3 years ago by mark.ziemann1.2k
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