Understanding heat map of splicing profiling

Question

[katja.cop.07]

Hi,

I have trouble with understanding the heat map in Figure 4A in this article. Does it mean inclusion and skipping of alternative exons or whole genes on the right?

http://www.cell.com/cell-reports/pdfExtended/S2211-1247(15)00141-2

Thank you

[katja.cop.07]

How did they get values for each gene? If I understand it right, fold change is a ratio between expression of 2 isoforms for each gene. For example, whats the difference between Pdlim5 and Dysf? (Im a beginner as you see)

[Devon Ryan]

Presumably they give details in the supplemental methods.

Answer 1

[Devon Ryan]

It's talking about alternate exons. From page 4 of the PDF:

We recorded a sufficient number of splicing changes (more than ten counts with both isoforms, p < 0.05 among triplicate) among 70% of total surveyed splicing events in both WT and RBFox2-/- hearts at all three stages (Figure S3 ). In aggregate, we detected a total of 709 splicing switching events in 624 genes, among which 346 events exhibited enhanced exon skipping and 363 events displayed induced exon inclusion in response to RBFox2 ablation (Figure 4A; Table S2).