Question: How to annotate the nucleotide sequences in terms of KEGG Orthology (KO)?
1
gravatar for bdpunith
4.0 years ago by
bdpunith10
India
bdpunith10 wrote:

I have a RNA-Seq data in which I have to construct the KEGG Pathway for DGE between two samples. I also have nucleotide sequences (contigs) in the fasta format (.fa) file. Kindly tell me how to assign the KO IDs to these nucleotide sequences. Also help me in construction of pathways either using KEGG, Cytoscape or any other pathway network builder software/server. . 

next-gen • 3.6k views
ADD COMMENTlink modified 4.0 years ago by Nari870 • written 4.0 years ago by bdpunith10
2
gravatar for dago
4.0 years ago by
dago2.5k
Germany
dago2.5k wrote:

try BLASTKOALA http://www.kegg.jp/blastkoala/

or KEGGMAPPER http://www.kegg.jp/kegg/tool/annotate_sequence.html

ADD COMMENTlink written 4.0 years ago by dago2.5k
1
gravatar for Nari
4.0 years ago by
Nari870
United States
Nari870 wrote:

kaas automatic server can do that. check here.

ADD COMMENTlink written 4.0 years ago by Nari870
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