I carried out RNAseq and smallRNAseq on WT, Mutant1, Mutant2 and Double Mutant M1M2 with NO replicates at two different temperatures.
Will the absence of Replicates affect my data analysis?
Can anyone advise how can I correlated the Transcriptome data (RNAseq) with data generated from smallRNAseq (ncRNAs)?
How can I run GO and Pathway enrichment analysis?
Is cummeRbund going to help me make sense of the data?