Dear all,
I have two closely related species (A and B) in the same genus. They are worms. I have assembled genomes of these two species. I made blast database on unix using 248 CORE genes (protein sequences) from CEGMA website. I have a protein fasta file of species A. My questions are:
- Should I find first a core gene in genome A, then find the same core gene in genome B
- Or should I find a core gene in genome A , and then Should I find the gene in the genome A which is close to the core gene in genome B?
- How to find nucleotide sequence of core genes in genome files?
- How to convert DNA sequence of a genome file into protein sequence?
Thanks
I have run CEGMA for two species. yes I have a protein fasta file given by my supervisor. I don't know how to convert a genome DNA sequence file into protein file.
Thank you for your help.