generation of contigs from sanger sequencing
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5.9 years ago

Hi,

I have a lot of sanger sequences to assemble into the contigs. To be more precise, I used 50 samples for 3 primer pairs that yielded me 300 sequences. I want to make contig for each sample of each primer pair separately. Eventually, I will end up with 150 contigs generated from forward and reverse sequences of each sample.

I know there are programs like CAP3 to generate contigs. But, is there any way to generate multiple contigs simultaneously ? In my case, 150 contigs at once ?

Looking forward for the answers!!

Thanks in advance

sequence contig • 2.1k views
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Entering edit mode
5.9 years ago

Try thisĀ http://egassembler.hgc.jp/

Although you cannot decide the number of contigs. It depends upon your data

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