generation of contigs from sanger sequencing

Question

[jasbadesha27]

Hi,

I have a lot of sanger sequences to assemble into the contigs. To be more precise, I used 50 samples for 3 primer pairs that yielded me 300 sequences. I want to make contig for each sample of each primer pair separately. Eventually, I will end up with 150 contigs generated from forward and reverse sequences of each sample.

I know there are programs like CAP3 to generate contigs. But, is there any way to generate multiple contigs simultaneously? In my case, 150 contigs at once?

Looking forward for the answers!

Thanks in advance

Answer 1

[Antonio R. Franco]

Try this http://egassembler.hgc.jp/

Although you cannot decide the number of contigs. It depends upon your data