Question: MPprimes vs. PCRTiler for PCR Multiplexing
2
gravatar for rrcutler
4.4 years ago by
rrcutler120
United States
rrcutler120 wrote:

I was trying to write a script to design primers for PCR multiplexing when I ran upon these programs that were already made for the same reason. However, my question is which seems to be the better pick? From my review, they both use primer3 to design the primers, although MPprimer uses MFEprimer to further analyze primer interactions while PCRTiler uses BlastN.

 

Links to multiplex PCR primer design:

http://biocompute.bmi.ac.cn/MPprimer/

http://pcrtiler.alaingervais.org:8080/PCRTiler/index.jsp

target rna-seq pcr • 2.4k views
ADD COMMENTlink modified 4.3 years ago by Wubin Qu160 • written 4.4 years ago by rrcutler120
1

Any update to this topid?

ADD REPLYlink written 15 months ago by Shicheng Guo7.8k
2
gravatar for Wubin Qu
4.3 years ago by
Wubin Qu160
China
Wubin Qu160 wrote:

You can write a pipeline for design multiplex PCR primers with primer pick software (e.g. Primer3), primer specific checking program (e.g. MFEprimer) and primer secondary structure checking program (e.g. use ntthal to check  self-dimer, cross-dimer, hairpin).

 

I am the author of MFEprimer and MPprimer.

ADD COMMENTlink written 4.3 years ago by Wubin Qu160
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