How to get the coding sequence of each isoform of a gene?
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8.9 years ago
Ömer An ▴ 260

I want to know if my siRNA sequence is present in all the isoforms of my gene to make sure that it targets and knock outs my gene for all possible isoforms. For this, I need the coding nucleotide sequence of each isoform to do the intersection. CCDS gives only the consensus sequence, UCSC is not straightforward to get what I want, so I am looking for an elegant way of doing this. Any idea?

isoform sequence • 4.3k views
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8.9 years ago
Emily 23k

You could look at the transcript comparison view in Ensembl.

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8.9 years ago
pld 5.1k

Ensembl does a good job of organizing isoforms: http://useast.ensembl.org/Homo_sapiens/Gene/Summary?db=core;g=ENSG00000109971;r=11:123057489-123063230;redirect=no

For each gene you can look at the transcript table and it will list all of the isoforms. You might be able to automate this through biomart.

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