I'm trying to do tblastn (ncbi-blast2-2-30+ version) using fasta sequences of protein as a query and my de novo assembled transcriptome as a nucleotide database as following:
./tblastn -query arapr.fasta -db database1 -out tblastn1.txt -evalue 1e-3 -max_target_seqs 1 -num_threads 5 -outfmt 6
but the tblastn was completed with the following warning:
"Warning: lcl|Query_18905 AT1G56045.1 pacid=19656851 transcript=AT1G56045.1 locus=AT1G56045 ID=AT1G56045.1.TAIR10 annot-version=TAIR10: Warning: Could not calculate ungapped Karlin-Altschul parameters due to an invalid query sequence or its translation. Please verify the query sequence(s) and/or filtering options"
Could anybody please help me to resolve the warning. Please don't refer me to the similar thread on biostar, I see it, but not useful for me.
Thanks for your help in advance.