I did de novo assembly with the RNAseq reads and then blast all the assembled sequences. Then I assigned the blasted gene names to each of my assembled sequences and stored them in a fasta file. Now I'd like to do some gene annotation, e.g. functional analysis, enrichment analysis..., and make some graphs.
Which software should I use, blast2go, DAVID or something else? Are there any good free software I can use? Can I use the fasta file as my input or only the gene names are sufficient?
I'm no bioinformaticists. Please help me.
Is it free?
Yes, open source and free!
Which would be recommended between Trinotate and Annocript?